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[Aviva] NEFL ELISA Kit (Human)

Cat-No. OKCD08589

Aviva Systems Biology는 기초 연구부터 전임상, 신약 개발, 진단 워크플로우 전반에서 사용할 수 있도록

폴리클로널·모노클로날 항체, 재조합 단백질, ELISA 키트, 그리고 맞춤형 단백질·항체 개발 서비스를 제공합니다.

제품 설명

NEFL ELISA Kit (Human)

제품 번호

OKCD08589

제품 특징

Product Info

Product Info

Predicted Species Reactivity

Homo sapiens|Human

Application

ELISA-Sandwich

ELISA Kit Detection Method

Colorimetric

ELISA Kit Duration

ELISA Kit Principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neurofilament, Light Polypeptide (NEFL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neurofilament, Light Polypeptide (NEFL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neurofilament, Light Polypeptide (NEFL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Neurofilament, Light Polypeptide (NEFL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ELISA Kit Range

15.6-1,000pg/mL

ELISA Kit Reproducibility

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neurofilament, Light Polypeptide (NEFL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neurofilament, Light Polypeptide (NEFL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

ELISA Kit Component

Component	Amount
NEFL Microplate	96 Wells (12 x 8 Well strips)
NEFL Lyophilized Standard	2
100X NEFL HRP-Detector Anitbody	1 x 120 uL
100X Avidin-HRP Conjugate	1 x 120 uL
Standard Diluent	1 x 20 mL
Detector Antibody Diluent	1 x 12 mL
Conjugate Diluent	1 x 12 mL
30X Wash Buffer	1 x 20 mL
TMB Substrate	1 x 9 mL
Stop Solution	1 x 6 mL

Reconstitution and Storage

2°C to 8°C|-20°C

Sample Type

Serum, plasma, cerebrospinal fluid and other biological fluids

Sensitivity

< 6.2pg/mL

Target Info

Gene Symbol	NEFL
Gene Full Name	neurofilament light chain
Alias Symbols	68 kDa neurofilament protein;CMT1F;CMT2E;CMTDIG;light molecular weight neurofilament protein;neurofilament light polypeptide;neurofilament protein, light chain;neurofilament subunit NF-L;neurofilament triplet L protein;neurofilament, light polypeptide 68kDa;NF68;NFL;NF-L;PPP1R110;protein phosphatase 1, regulatory subunit 110.
NCBI Gene Id	4747
Protein Name	Neurofilament light polypeptide
Description of Target	Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
Uniprot ID	P07196
Protein Accession #	NP_006149.2
Nucleotide Accession #	NM_006158.4