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Biotechrabbit

[Biotechrabbit] LYO-ready Exonuclease III, 100 U/µl

Cat-No. BR1500101


Biotechrabbit은 바이오의약품 및 생명과학 연구를 위한 고급 실험 재료와 서비스를 제공하는 회사입니다.



제품 설명

 

LYO-ready Exonuclease III, 100 U/µl



제품 번호


BR1500101 - 10,000 Units

BR1500102 - 25,000 Units


제품 특징


Features

  • Low glycerol content for further use in lyophilization
  • High exonuclease activity for efficient removal of nucleotides
  • Exceptional purity suitable for the most sensitive applications

Applications

  • Recombinase Polymerase Amplification (RPA)
  • Other isothermal amplification techniques (e.g., RCA)
  • Cloning of PCR products
  • Unidirectional nested deletions (3'→5' exonuclease activity)
  • Generation of a single-stranded template for dideoxy
  • Sequencing of DNA
  • Production of intermediate products for site-directed mutagenesis
  • Preparation of strand-specific probes


biotechrabbit™ LYO-ready Exonuclease III is a highly processive exonuclease that can continuously remove multiple nucleotides without dissociating from the DNA strand. This 31 kDa enzyme removes nucleotides from the 3' end of double-stranded DNA in a stepwise manner and continues to move in the 3'→5' direction. In contrast to other exonucleases that operate sequentially at one site, Exonuclease III exhibits the ability to simultaneously target multiple DNA molecules or multiple sites within a single DNA molecule. This capability enables the enzyme to rapidly and cooperatively remove nucleotides from DNA strands.

In addition to its exonuclease activity, the enzyme also exhibits RNase H, 3' DNA phosphatase, and apurinic DNA endonuclease activities. This enzymatic activity finds practical applications in DNA sequencing, removal of unwanted DNA sequences, and the creation of precise DNA fragments for genetic engineering. biotechrabbit Exonuclease III is provided in a storage buffer containing 5 % glycerol, making it suitable for further use in lyophilization.








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Biotechrabbit - Official Distributor in South Korea "Morebio" 한국 공식 대리점 "모아바이오"

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