(주)모아바이오

Signosis

[Signosis] Custom Human Cytokine ELISA Strip

Cat-No. EA-1001-Cus_007247

Signosis는 생명 과학 연구 및 생물학적 데이터 분석을 위한 혁신적인 솔루션을 제공하는 글로벌 생명과학 기업입니다.

제품 설명

Custom Human Cytokine ELISA Strip

제품 번호

EA-1001-Cus_007247

제품 특징

Introduction

Cytokines are essential molecules that have crucial roles in many biological functions, including viral infection, inflammation, immunity, and hematopoiesis. Cytokines are produced by a variety of cell types in response to different stimuli. In addition, the expression of cytokine genes appear to be regulated by complex mechanisms. Expression of one cytokine gene could be regulated by other cytokines. Dysregulation of cytokine gene expression may be caused by chromosomal alterations or by infection of viruses that induce activation or inactivation of the expression machinery. Therefore, profiling of these cytokines is critical in understanding these biological functions. Signosis’ Custom Human ELISA Strip can quantitatively profile and measure 8 human cytokines.

Principle of the assay

Each well of the plate is coated with a specific capture antibody to detect its corresponding cytokine in the sample. Therefore, 8 different proteins can be measured simultaneously. The test sample reacts simultaneously with pairs of two antibodies, resulting in the human cytokines being sandwiched between the solid phase and enzyme-linked antibodies. After incubation, the wells are washed to remove unbound-labeled antibodies. The HRP substrate, TMB, is added to result in the development of a blue color. The reaction is then terminated with the addition of Stop Solution, resulting in a yellow color. The concentrations of oxidative stress cytokines are directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm. The expression levels of these cytokines can be quantitatively compared between samples.

Materialsprovided with the kit