Signosis는 Reporter Stable Cell Lines 제품군과 연관 키트들을 전문적으로 제공하는 미국의 제조업체 입니다.
제품 설명
DCFDA ROS Assay Kit (100 Tests)
제품 번호
EA-7001
제품 특징
The DCFDA ROS Assay kit utilizes 2’,7’ –dichlorofluorescin diacetate (DCFDA), a cell permeable reagent, to detect reactive oxygen species in live cells. DCFDA is capable of measuring hydroxyl, peroxyl, and other reactive oxygen species activity within the cell. When DCFDA enters the cell, it is deacetylated by cellular esterases into a non-fluorescent compound. Then, it is oxidized by reactive oxygen species into 2’, 7’ –dichlorofluorescein (DCF), which is highly fluorescent and can be detected by fluorescence spectroscopy at excitation and emission wavelengths of 485 nM and 535 nM, respectively.
For this assay, the cells are first stained with DCFDA. Then, the cells can be treated with compound(s) of interest and measured using a fluorescent plate reader.
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