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[Aviva] NEFL High-sensitivity ELISA kit (Mouse)

Cat-No. OKCD01381

Aviva Systems Biology는 기초 연구부터 전임상, 신약 개발, 진단 워크플로우 전반에서 사용할 수 있도록

폴리클로널·모노클로날 항체, 재조합 단백질, ELISA 키트, 그리고 맞춤형 단백질·항체 개발 서비스를 제공합니다.

제품 설명

NEFL High-sensitivity ELISA kit (Mouse)

제품 번호

OKCD01381

제품 특징

Product Info

Product Info

Predicted Species Reactivity

Mouse|Mus musculus

Application

ELISA-Comp

ELISA Kit Detection Method

Colorimetric

ELISA Kit Duration

ELISA Kit Linearity

Sample	1:2	1:4	1:8	1:16
Serum (n=5)	99-105%	83-97%	93-103%	94-102%
EDTA Plasma (n=5)	82-103%	81-96%	79-95%	82-102%
Heparin Plasma (n=5)	94-102%	87-96%	92-101%	90-97%

ELISA Kit Principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to High Sensitive Neurofilament, Light Polypeptide (NEFL) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled High Sensitive Neurofilament, Light Polypeptide (NEFL) and unlabeled High Sensitive Neurofilament, Light Polypeptide (NEFL) (Standards or samples) with the pre-coated antibody specific to High Sensitive Neurofilament, Light Polypeptide (NEFL). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of High Sensitive Neurofilament, Light Polypeptide (NEFL) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of High Sensitive Neurofilament, Light Polypeptide (NEFL) in the sample.

ELISA Kit Range

4.94-400pg/mL

ELISA Kit Recovery

Matrix	Recovery range (%)	Average(%)
Serum (n=5)	81-96	91
EDTA Plasma (n=5)	94-105	102
Heparin Plasma (n=5)	78-98	87

ELISA Kit Reproducibility

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Neurofilament, Light Polypeptide (NEFL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Neurofilament, Light Polypeptide (NEFL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

ELISA Kit Component

Component	Amount
Anti-Nefl Microplate	96 Wells (12 x 8 Well Strips)
Nefl Lyophilized Standard	2
100X Biotinylated Nefl Detector Antibody	120 uL
Avidin/HRP Conjugate	120 uL
Standard Diluent	1 x 20 mL
Detector Antibody Diluent	1 x 12 mL
Conjugate Diluent	1 x 12 mL
30X Wash Buffer	1 x 20 mL
TMB Substrate	1 x 9 mL
Stop Solution	1 x 6 mL

Reconstitution and Storage

2°C to 8°C|-20°C

Sample Type

Serum, plasma, cerebrospinal fluid and other biological fluids

Sensitivity

1.95 pg/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of Neurofilament, Light Polypeptide (NEFL). No significant cross-reactivity or interference between Neurofilament, Light Polypeptide (NEFL) and analogues was observed.

Assay Info

Assay Methodology: Quantitative Sandwich ELISA

Target Info

Gene Symbol	Nefl
Gene Full Name	neurofilament, light polypeptide
Alias Symbols	68 kDa neurofilament protein;AI847934;CMT2E;N;neurofilament light polypeptide;neurofilament triplet L protein;NF;NF6;NF68;Nfl;NF-L.
NCBI Gene Id	18039
Protein Name	Neurofilament light polypeptide
Description of Target	Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
Uniprot ID	P08551
Protein Accession #	NP_035040.1
Nucleotide Accession #	NM_010910.1