Bioassay Systems는 생화학 및 생물학적 분석 솔루션을 제공하는 기업으로, 

다양한 생물학적 실험 및 시험을 위한 고품질 키트와 서비스를 개발하고 제공합니다.

제품 설명 

QuantiFluo™ Chymotrypsin Inhibitor Assay Kit

제품 번호

DCTI-100

제품 특징

Application

• For evaluation of drugs and screening of potential inhibitors to chymotrypsin proteases.

Key Features

• Safe. Non-radioactive assay.
• Homogeneous and convenient. Homogenous “Mix-incubate-measure” type assay. No wash and reagent transfer steps are involved.
• Robust and High-throughput. Can be readily automated to assay thousands of samples per day. Robust assay with a Z’ factor of >0.8.

Method

• FL485/530 nm

Samples

• Compounds that affect chymotrypsin protease activity.

Species

• All

Procedures

• 45 min

Size

• 100 tests in 96-well plate (Custom bulk sizes available upon request)

Shelf Life

• 6 months

Screening Services

• BioAssay Systems offers comprehensive Chymotrypsin lead discovery services, including compound library screening, inhibitor profiling, and high-throughput assays to identify potential drug candidates. We provide detailed analyses of compound activity, potency, and selectivity, supporting hit-to-lead and lead optimization efforts. Please contact us for more details at Service.

More Details

CHYMOTRYPSIN (EC 3.4.21.1) is a digestive, serine protease that hydrolyzes dietary proteins in many eukaryotic and prokaryotic organisms. Chymotrypsin predominantly cleaves peptide chains at the carboxyl side of the aromatic phenylalanine, tryptophan, and tyrosine amino acids. BioAssay System’s QuantiFluo™ Chymotrypsin Inhibitor Assay Kit uses a fluorescein isothiocyanate (FITC)-labeled synthetic substrate. The fluorescein label is highly quenched. Upon digestion by chymotrypsin present in the sample, the substrate is cleaved into smaller peptides, which abolishes the quenching of the fluorescence label. The fluorescence or fluorescence polarization (FP) of the FITC-labeled fragments is measured at λ_ex/em = 485/530 nm. Inhibition is determined by the decrease in fluorescence.

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ASSAY KITS & REAGENTS

• Agriculture & Environment
• Blood & Urine Chemistry
• Cations & Anions
• Diabetes & Obesity
• Enzyme Activity
• Food & Beverage Analysis
• HTS Reagents & Kits
• Inhibitor HTS Kits
• Metabolism
• Oncology
• Other Reagents
• Oxidative Stress
• Quick Test Strips
• Signal Transduction

EQUIPMENT & ACCESSORIES

• Handheld & Plate Readers
• Microtiter Plates

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