T7 RNA Polymerase ELISA Kit

제품 번호

L01110

제품 특징

Product Introduction

Product Description

The kit is used for detection and quantitative determination of T7 RNA Polymerase in the samples. T7 RNA Polymerase ELISA kit uses the double antibody sandwich method: The micro plate has been pre-coated with anti-T7 RNA polymerase antibody. The sample is added and incubated with horseradish peroxidase (HRP) labeled antibody to form antibody-antigen-enzyme-labeled antibody complex. After washing, then TMB substrate solution is added and catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The depth of the color is positively correlated with the T7 RNA polymerase content in the sample. The absorbance (OD value) is measured at 450 nm.

Linearity Range

0.25-32 ng/mL

Detection Range

Lower limit of detection: ＜0.1 ng/mL
Lower limit of quantitation: 0.25 ng/mL

Precision

Intra-assay CV≤10%, Inter-assay CV≤15%

Recovery

80%-120%

Specificity

only specific recognition of T7 RNA polymerase, other IVT tool enzymes do not interfere with the measurements.

Storage

Unopened kit should be stored at 2-8°C for stability in shelf life.

Kit Contents

Component	Quantity/Size
Capture Plate	1 plate
Standard Stock	50 μL
Enzyme Conjugate (100×)	80 μL
Sample Dilution Buffer	30 mL
Enzyme Conjugate Dilution Buffer	8 mL
Wash Solution (10×)	30 mL
Substrate Solution	12 mL
Stop Solution	6 mL
Plate Sealer	4 pieces

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Genscript - Official Distributor in South Korea "Morebio" 한국 공식 대리점 "모아바이오"

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Genscript

[Genscript] T7 RNA Polymerase ELISA Kit

Cat-No. L01110

T7 RNA Polymerase ELISA Kit