His Tag ELISA Detection Kit

제품 번호

L00436

제품 특징

Note

L00436 interferes with IgG in mouse/rat serum and is not suitable for detecting mouse/rat serum samples.

Product Introduction

Product Description

GenScript His Tag ELISA Detection Kit, a 1.5 hour competition ELISA, is developed for rapid and high throughput detection of His-tagged proteins. There are several potential usages:
●Quickly identify the presence of His-tagged proteins in samples.
●Optimize protein expression by monitoring the His-tagged proteins level.
●High throughput screening of stable cell lines expressing His-tagged proteins.

Detection Range

1 ng/ml~729 ng/ml

Sensitivity

1 ng/ml His-tagged proteins

Test Samples

N-terminal/C-terminal/internal His-tagged proteins4 x His/5 x His/6 x His-tagged proteins
Mammalian, yeast and bacteria cell lysates or cell supernatant

Conveniency

Provide all reagents required for test
Complete test within 1.5 hours

Reagents compatibility

Tolerable with various reagents at certain concentration

Storage

The unopened kit is stable for 12 months if stored at 2-8 °C and the opened kit may be stable for 1 month at 2-8 °C. Do not freeze the kit.

Kit Contents

Component	Quantity	Part. No
His Tag Plate	1 plate (8 wells × 12 strips)	436-80
Anti-His Monoclonal Antibody	6 ml	436-20
Antibody Tracer	12 ml	436-30
His Tag Standards (0,1,3,9,27,81,243,729 ng/ml)	1 ml per each standard	436-11,12,13,14,15,16,17,18
Assay Diluent	60 ml	436-60
20 × Wash Solution	40 ml	B-A1-70
TMB Solution	12 ml	B-A1-40
Stop Solution	6 ml	B-A1-50
His Tag Standard Stock (10 μg/ml)	500 μl	436-10
Plate Sealer	2	N/A

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Products

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Genscript

[Genscript] His Tag ELISA Detection Kit

Cat-No. L00436

His Tag ELISA Detection Kit