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제품 설명
Human Melatonin Receptor 1A (MT1) ACTOne Stable Cell Line
제품 번호
CL-11-MT1
제품 특징
Detailed Description
CL-11-MT1 | |
Name | Melatonin Receptor 1A (MT1) ACTOneTM Stable Cell Line |
Description | The ACTOne™ MT1 stable cell line is a HEK-293 CNG derivative engineered to express recombinant human melatonin receptor 1A (MT1/MTNR1A). MT1 is primarily coupled to Gi/o proteins; receptor activation inhibits adenylyl cyclase and reduces intracellular cAMP. The assay allows both end-point and kinetic measurement of intracellular cAMP changes with a FLIPR or a fluorescence microplate reader. |
Application | cAMP dependent human MT1 cell-based assay; Cell-based high-throughput screening (HTS) of human MT1 agonists/antagonists |
Size | 1 mL (frozen cells; 1x 106 cells before freezing) |
Detection | FlexStation or Microplate reader |
Detection | ==>> DATA SHEET ==>> MSDS |
Melatonin Receptor 1A (MTNR1A), commonly known as MT1, is a Class A G protein-coupled receptor (GPCR) activated by the endogenous hormone melatonin. MT1 primarily couples to Gi/o proteins, resulting in inhibition of adenylyl cyclase activity and reduced intracellular cAMP levels. The receptor is expressed in the suprachiasmatic nucleus and other regions of the central nervous system, as well as in several peripheral tissues, where it contributes to circadian rhythm regulation, sleep initiation, endocrine function, and seasonal physiological responses.
MT1 is an important therapeutic target for insomnia, circadian rhythm disorders, mood disorders, and other conditions associated with disrupted melatonin signaling. Pharmacological studies commonly use melatonin receptor agonists and antagonists to investigate receptor activation, selectivity, desensitization, and downstream signaling.
eEnzyme's Human MT1 ACTOne™ Stable Cell Line is a HEK-293 CNG derivative engineered to express recombinant human MTNR1A. Activation of the Gi/o-coupled receptor decreases intracellular cAMP, which can be measured using the ACTOne™ fluorescent reporter system. The assay supports both endpoint and kinetic measurements and is compatible with FLIPR, FlexStation, and fluorescence microplate readers.
MT1 is a major mediator of melatonin signaling and plays an important role in synchronizing physiological processes with the light-dark cycle. Through Gi/o-mediated inhibition of cAMP production, MT1 influences neuronal activity, sleep timing, endocrine rhythms, and seasonal biological responses. Altered MT1 expression or signaling has been investigated in sleep disorders, circadian disruption, metabolic dysfunction, and neuropsychiatric conditions.
Because of its central role in melatonin-mediated physiology, MT1 remains an important target for chronobiology, neuroscience, and GPCR-focused drug discovery. The MT1 ACTOne™ Stable Cell Line provides a reliable platform for studying receptor function and evaluating compounds that modulate melatonin signaling.
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