eEnzyme는 다양한 생물에서 단백질 발현을 최적화하는 코돈 최적화 서비스를 제공하는 미국의 Biological 기업입니다.
제품 설명
Melatonin Receptor 1B (MT2) ACTOne Stable Cell Line
제품 번호
CL-11-MT2
제품 특징
Detailed Description
CL-11-MT2 | |
Name | Melatonin Receptor 1B (MT2) ACTOneTM Stable Cell Line |
Description | The ACTOne™ MT2 stable cell line is a HEK-293 CNG derivative engineered to express recombinant human melatonin receptor 1B (MT2/MTNR1B). MT2 is primarily coupled to Gi/o proteins; receptor activation inhibits adenylyl cyclase and reduces intracellular cAMP. The assay allows both end-point and kinetic measurement of intracellular cAMP changes with a FLIPR or a fluorescence microplate reader. |
Application | cAMP dependent human MT2 cell-based assay; Cell-based high-throughput screening (HTS) of human MT1 agonists/antagonists |
Size | 1 mL (frozen cells; 1x 106 cells before freezing) |
Detection | FlexStation or Microplate reader |
Detection | ==>> DATA SHEET ==>> MSDS |
Melatonin Receptor 1B (MTNR1B), commonly known as MT2, is a Class A G protein-coupled receptor (GPCR) activated by the endogenous hormone melatonin. MT2 primarily couples to Gi/o proteins, resulting in inhibition of adenylyl cyclase activity and reduced intracellular cAMP levels. The receptor is expressed in the central nervous system, retina, and several peripheral tissues, where it contributes to circadian rhythm regulation, sleep–wake timing, retinal physiology, and metabolic homeostasis.
MT2 is an important therapeutic target for insomnia, circadian rhythm disorders, mood disorders, and other conditions associated with altered melatonin signaling. MTNR1B has also attracted interest in metabolic research because genetic variation near or within the gene has been associated with fasting glucose regulation and type 2 diabetes risk.
eEnzyme's Human MT2 ACTOne™ Stable Cell Line is a HEK-293 CNG derivative engineered to express recombinant human MTNR1B. Activation of the Gi/o-coupled receptor decreases intracellular cAMP, which can be measured using the ACTOne™ fluorescent reporter system. The assay supports endpoint and kinetic measurements and is compatible with FlexStation, FLIPR, and fluorescence microplate readers.
MT2 is an important mediator of melatonin signaling and contributes to the synchronization of physiological processes with environmental light–dark cycles. Through Gi/o-mediated inhibition of cAMP production, MT2 influences circadian phase regulation, sleep timing, retinal signaling, neuroendocrine function, and metabolic processes. Altered MT2 expression or activity has been investigated in sleep and circadian disorders, impaired glucose regulation, and neuropsychiatric conditions.
Because of its roles in chronobiology, nervous system function, and metabolism, MT2 remains an important target for GPCR pharmacology and drug discovery. The MT2 ACTOne™ Stable Cell Line provides a reliable platform for studying receptor function and evaluating compounds that modulate melatonin signaling.
eEnzyme의 모든 제품을 만나보세요!
Infectious Disease Research Tools
Molecular & Cell Biology Reagents
Proteins for Advanced Medicine
eEnzyme - Official Distributor in South Korea "Morebio" 한국 공식 대리점 "모아바이오"
MSDS
온라인 견적
