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NEFL High-sensitivity ELISA kit (Human)

Cat-No. OKCD01380

Aviva Systems Biology는 기초 연구부터 전임상, 신약 개발, 진단 워크플로우 전반에서 사용할 수 있도록

폴리클로널·모노클로날 항체, 재조합 단백질, ELISA 키트, 그리고 맞춤형 단백질·항체 개발 서비스를 제공합니다.

제품 설명

NEFL High-sensitivity ELISA kit (Human)

제품 번호

OKCD01380

제품 특징

Product Info

Product Info

Predicted Species Reactivity

Homo sapiens|Human

Application

ELISA-Sandwich

ELISA Kit Detection Method

Colorimetric

ELISA Kit Duration

ELISA Kit Linearity

Sample	1:2	1:4	1:8	1:16
Serum (n=5)	88-96%	80-104%	95-105%	97-105%
EDTA Plasma (n=5)	78-101%	91-101%	87-104%	86-101%
Heparin Plasma (n=5)	92-102%	79-99%	82-103%	88-101%

ELISA Kit Principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Neurofilament, Light Polypeptide (NEFL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Neurofilament, Light Polypeptide (NEFL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Neurofilament, Light Polypeptide (NEFL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Neurofilament, Light Polypeptide (NEFL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ELISA Kit Range

1.56-100pg/mL

ELISA Kit Recovery

Matrix	Recovery range (%)	Average(%)
Serum (n=5)	78-90	86
EDTA Plasma (n=5)	92-105	101
Heparin Plasma (n=5)	89-97	94

ELISA Kit Reproducibility

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Neurofilament, Light Polypeptide (NEFL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Neurofilament, Light Polypeptide (NEFL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

ELISA Kit Component

Component	Amount
Anti-NEFL Microplate	96 Wells (12 x 8 Well Strips)
NEFL Lyophilized Standard	2
100X Biotinylated NEFL Detector Antibody	120 uL
Avidin/HRP Conjugate	120 uL
Standard Diluent	1 x 20 mL
Detector Antibody Diluent	1 x 12 mL
Conjugate Diluent	1 x 12 mL
30X Wash Buffer	1 x 20 mL
TMB Substrate	1 x 9 mL
Stop Solution	1 x 6 mL

Reconstitution and Storage

2°C to 8°C|-20°C

Sample Type

Serum, plasma, cerebrospinal fluid and other biological fluids

Sensitivity

< 0.61 pg/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of Neurofilament, Light Polypeptide (NEFL). No significant cross-reactivity or interference between Neurofilament, Light Polypeptide (NEFL) and analogues was observed.

Publications

Mu-Hong Chen, Yu-Li Liu, Hsiang-Wei Kuo, Shih-Jen Tsai, Ju-Wei Hsu, Kai-Lin Huang, Pei-Chi Tu, Ya-Mei Bai. Neurofilament Light Chain Is a Novel Biomarker for Major Depression and Related Executive Dysfunction.. Int J Neuropsychopharmacol. 25, 99-105 (2022). 34637515

Jedd Pratt, Giuseppe De Vito, Ricardo Segurado, Ludmilla Pessanha, Jackie Dolan, Marco Narici, Colin Boreham. Plasma neurofilament light levels associate with muscle mass and strength in middle-aged and older adults: findings from GenoFit.. J Cachexia Sarcopenia Muscle. 13, 1811-1820 (2022). 35415973

Jedd Pratt, Ludmilla Pessanha, Marco Narici, Colin Boreham, Giuseppe De Vito. Handgrip strength asymmetry as a new biomarker for sarcopenia and individual sarcopenia signatures.. Aging Clin Exp Res. 35, 2563-2571 (2023). 37658983

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Assay Info

Assay Methodology: Quantitative Sandwich ELISA

Target Info

Gene Symbol	NEFL
Gene Full Name	neurofilament light chain
Alias Symbols	68 kDa neurofilament protein;CMT1F;CMT2E;CMTDIG;light molecular weight neurofilament protein;neurofilament light polypeptide;neurofilament protein, light chain;neurofilament subunit NF-L;neurofilament triplet L protein;neurofilament, light polypeptide 68kDa;NF68;NFL;NF-L;PPP1R110;protein phosphatase 1, regulatory subunit 110.
NCBI Gene Id	4747
Protein Name	Neurofilament light polypeptide
Description of Target	Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
Uniprot ID	P07196
Protein Accession #	NP_006149.2
Nucleotide Accession #	NM_006158.4