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[Aviva] Dihydrotestosterone ELISA Kit

Cat-No. OKEH02531

Aviva Systems Biology는 기초 연구부터 전임상, 신약 개발, 진단 워크플로우 전반에서 사용할 수 있도록

폴리클로널·모노클로날 항체, 재조합 단백질, ELISA 키트, 그리고 맞춤형 단백질·항체 개발 서비스를 제공합니다.

제품 설명

Dihydrotestosterone ELISA Kit

제품 번호

OKEH02531

제품 특징

Product Info

Product Info

Predicted Species Reactivity

All Species

Application

ELISA-Comp

ELISA Kit Detection Method

Colorimetric, OD450 nm

ELISA Kit Duration

~ 3 Hours

ELISA Kit Principle

Aviva Systems Biology Dihydrotestosterone ELISA Kit (OKEH02531) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Dihydrotestosterone antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Dihydrotestosterone and incubated. The Dihydrotestosterone found in the sample or standards competes with the biotinylated Dihydrotestosterone for limited binding sites on the immobilized anti-Dihydrotestosterone antibody. Excess unbound biotinylated Dihydrotestosterone and sample or standard Dihydrotestosterone is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Dihydrotestosterone captured in the well and inversely proportional to the amount of Dihydrotestosterone which was contained in the sample or standard.

ELISA Kit Range

0.156-10ng/mL

ELISA Kit Recovery

Mean recovery when spiking into Serum and Plasma = 96%

ELISA Kit Reproducibility

Mean Intra-assay CV%: < 4.2% (n = 20)
Mean Inter-assay CV%: < 7.8% (n = 20)

ELISA Kit Component

Component	Amount
Anti-Dihydrotestosterone Microplate	96 Wells (12 x 8 Well strips)
Dihydrotestosterone Lyophilized Standard	2 x 10 ng
Sample Diluent	1 x 20 mL
100X Dihydrotestosterone-Biotin Complex	1 x 60 uL
100X Avidin-HRP Conjugate	1 x 120 uL
Biotin Complex Diluent	1 x 12 mL
Conjugate Diluent	1 x 12 mL
25X Wash Buffer	1 x 30 mL
TMB Substrate	1 x 10 mL
Stop Solution	1 x 10 mL

Additional Information

Target Abbr: DHT
Target Name: Dihydrotestosterone

Pubchem: 10635

Chemical formula: C19H30O2

Reconstitution and Storage

Store as indicated in product manual.

Sample Type

Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids

Sensitivity

0.058 ng/mL

Specificity

Natural and recombinant General Dihydrotestosterone

Publications

Raphael Serruya, Yehoshua Maor. Hair growth-promotion effects at the cellular level and antioxidant activity of the plant-based extract Phyllotex.. Heliyon. 7, e07888 (2021). 34522800

Sung-Ok Kim, Arin Choi, Hee-Hwan Lee, Jae-Yong Lee, Sang Jae Park, Byung-Hak Kim. Purple corn extract improves benign prostatic hyperplasia by inhibiting 5 alpha-reductase type 2 and inflammation in testosterone propionate-induced rats.. Front Pharmacol. 15, 1485072 (2025). 39830338

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Assay Info

Assay Methodology: Quantitative Competitive ELISA

Target Info

Alias Symbols	DHT, Dihydrotestosterone, DHT
Protein Name	Dihydrotestosterone
Description of Target	Dihydrotestosterone (5alpha-Dihydrotestosterone, commonly abbreviated to DHT) is an androgen or male sex hormone. The enzyme 5alpha-reductase synthesises DHT in the prostate, testes, hair follicles, and adrenal glands. This enzyme reduces the 4,5 double-bond of the hormone testosterone.