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[Aviva] RIPK1 ELISA Kit (Rat)

Cat-No. OKCD00437

Aviva Systems Biology는 기초 연구부터 전임상, 신약 개발, 진단 워크플로우 전반에서 사용할 수 있도록

폴리클로널·모노클로날 항체, 재조합 단백질, ELISA 키트, 그리고 맞춤형 단백질·항체 개발 서비스를 제공합니다.

제품 설명

RIPK1 ELISA Kit (Rat)

제품 번호

OKCD00437

제품 특징

Product Info

Product Info

Predicted Species Reactivity

Rat|Rattus norvegicus

Application

ELISA-Sandwich

ELISA Kit Detection Method

Colorimetric

ELISA Kit Duration

ELISA Kit Principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Receptor Interacting Serine Threonine Kinase 1 (RIPK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Receptor Interacting Serine Threonine Kinase 1 (RIPK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Receptor Interacting Serine Threonine Kinase 1 (RIPK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Receptor Interacting Serine Threonine Kinase 1 (RIPK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ELISA Kit Range

0.156-10ng/mL

ELISA Kit Reproducibility

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Receptor Interacting Serine Threonine Kinase 1 (RIPK1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Receptor Interacting Serine Threonine Kinase 1 (RIPK1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

ELISA Kit Component

Component	Amount
Anti-Ripk1 Microplate	96 Wells (12 x 8 Well Strips)
Ripk1 Lyophilized Standard	2 x 10 ng
100X Biotinylated Ripk1 Detector Antibody	120 uL
Avidin/HRP Conjugate	120 uL
Standard Diluent	1 x 20 mL
Detector Antibody Diluent	1 x 12 mL
Conjugate Diluent	1 x 12 mL
30X Wash Buffer	1 x 20 mL
TMB Substrate	1 x 9 mL
Stop Solution	1 x 6 mL

Reconstitution and Storage

-20°C|2°C to 8°C

Sample Type

Tissue homogenates, cell lysates and other biological fluids

Sensitivity

< 0.055 ng/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of Receptor Interacting Serine Threonine Kinase 1 (RIPK1). No significant cross-reactivity or interference between Receptor Interacting Serine Threonine Kinase 1 (RIPK1) and analogues was observed.

Publications

Zhuoheng Li, Juan Li, Shuli He, Jun Chen, Chengjun Deng, Jintao Duan. Ellagic Acid Modulates Necroptosis, Autophagy, Inflammations, and Stress to Ameliorate Nonalcoholic Liver Fatty Disease in a Rat Model.. Food Sci Nutr. 13, e4694 (2025). 39830906

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Assay Info

Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

Gene Symbol	Ripk1
Gene Full Name	receptor interacting serine/threonine kinase 1
Alias Symbols	receptor (TNFRSF)-interacting serine-threonine kinase 1, receptor-interacting serine/threonine-protein kinase 1, RIP.
NCBI Gene Id	306886
Protein Name	Receptor (TNFRSF)-interacting serine-threonine kinase 1 (Predicted)
Description of Target	Receptor (TNFRSF)-interacting serine-threonine kinase 1 (Predicted)
Uniprot ID	D3ZYL0
Protein Accession #	NP_001100820
Nucleotide Accession #	NM_001107350.1
Protein Size (# AA)	658