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[Aviva] VEGFC ELISA Kit (Mouse) : 96 Wells

Cat-No. OKEH00240

Aviva Systems Biology는 기초 연구부터 전임상, 신약 개발, 진단 워크플로우 전반에서 사용할 수 있도록

폴리클로널·모노클로날 항체, 재조합 단백질, ELISA 키트, 그리고 맞춤형 단백질·항체 개발 서비스를 제공합니다.

제품 설명

VEGFC ELISA Kit (Mouse) : 96 Wells

제품 번호

OKEH00240

제품 특징

Product Info

Predicted Species Reactivity

Mouse|Mus musculus

Application

ELISA-Sandwich

ELISA Kit Detection Method

Colorimetric, OD450 nm

ELISA Kit Duration

~ 3 Hours

ELISA Kit Principle

Aviva Systems Biology VEGFC ELISA Kit (Mouse) (OKEH00240) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for VEGFC has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for VEGFC is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample VEGFC captured in well.

ELISA Kit Range

15.6-1000pg/mL

ELISA Kit Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 106%

ELISA Kit Reproducibility

Mean Intra-assay CV%: < 6.3% (n = 20)
Mean Inter-assay CV%: < 10.6% (n = 20)

ELISA Kit Component

Component	Amount
VEGFC Microplate	96 Wells (12 x 8 Well strips)
VEGFC Lyophilized Standard	2
Sample Diluent	1 x 20 mL
100X Biotinylated VEGFC Detector Antibody	1 x 120 uL
100X Avidin-HRP Conjugate	1 x 120 uL
Detector Antibody Diluent	1 x 12 mL
Conjugate Diluent	1 x 12 mL
25X Wash Buffer	1 x 30 mL
TMB Substrate	1 x 10 mL
Stop Solution	1 x 10 mL

Reconstitution and Storage

Store as indicated in product manual.

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Sensitivity

7.9 pg/mL

Specificity

Natural and recombinant Mouse Vascular endothelial growth factor C

Publications

Shoko Hasegawa, Toshiaki Nakano, Kumiko Torisu, Akihiro Tsuchimoto, Masahiro Eriguchi, Naoki Haruyama, Kosuke Masutani, Kazuhiko Tsuruya, Takanari Kitazono. Vascular endothelial growth factor-C ameliorates renal interstitial fibrosis through lymphangiogenesis in mouse unilateral ureteral obstruction.. Lab Invest. 97, 1439-1452 (2017). 29083411

Jain Jeong, Shao-Jung Hsu, Daiki Horikami, Teruo Utsumi, Yilin Yang, Nikolai Arefyev, Xuchen Zhang, Shi-Ying Cai, James Boyer, Rolando Garcia-Milan, Masatake Tanaka, Matthew McConnell, Hui-Chun Huang, Yasuko Iwakiri. Liver Lymphatic Dysfunction as a Driver of Fibrosis and Cirrhosis Progression. bioRxiv. , (2025).

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Assay Info

Assay Methodology: Quantitative Sandwich ELISA

Target Info

Gene Symbol	Vegfc
Gene Full Name	vascular endothelial growth factor C
Alias Symbols	AW228853, flt4 ligand, flt4-L, vascular endothelial growth factor C, vascular endothelial growth factor-related protein, VEGF-, VEGF-C, VRP.
NCBI Gene Id	22341
Protein Name	Vascular endothelial growth factor C
Description of Target	Growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates KDR/VEGFR2 and FLT4/VEGFR3 receptors.
Uniprot ID	P97953
Protein Accession #	NP_033532.1
Nucleotide Accession #	NM_009506.2
Protein Size (# AA)	415
Molecular Weight	47 kDa