Species:
Chinese hamster (Cricetulus griseus)
Origin:
Flp-In derivate of CHO-K1 cell line (originated from an ovary biopsy of an adult Chinese hamster in 1957) which were transfected with the gene for human insulin receptor B (INSR) inserted in the vector pCDNA(TM)5-FRT-TO from Invitrogen
Biosafety level:
1, GMO-S1
Risk assessment:
genetically modified organism, according to German and European legislation allocated into group S1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
Morphology:
epithelioid-like cells growing adherently in monolayers;
image;
image;
imageMedium:
90% Ham´s F12 + 10% h.i. FBS + 1 mM L-glutamine + 0.3 mg/ml Hygromycin
Subculture:
seed out 0.5-1 x 106 cells/80cm2; split 1:4 to 1:6 once or twice a week using accutase; after thawing viability may decrease and cells may need a few days to recover
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30-40 hours
Harvest:
cell harvest of ca. 8-10 x 106 cells/80 cm2 flask
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in PCR assay
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Cricetulus griseus species.
Supplied as:
| Delivery form | | Prices |
|
Growing culture
(please inquire for exact delivery time) | | 1040,- € |
| Frozen culture | | 520,- € |
| DNA isolated from cell line (25 µg) | | 650,- € |
| DNA isolated from cell line (5 µg) | | 160,- € |
see price list
MSDS
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