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제품 설명

NAT-MAT® dPCR

제품 번호

58-0503

제품 특징

NAT-MAT® dPCR provides a lyophilized primer/probe mix and parallelly measures the gene expression of two cytokines – IL-1β (HEX™) and TNF-α (Cy5®) – and one housekeeping gene (FAM™) in a triplex PCR reaction. NAT-MAT® dPCR can be used in combination with NAT-MAT® Cells (Cat. No. 58-0501) and NAT-MAT® Extraction (Cat. No. 58-0502) to perform monocyte activation test (MAT) in order to detect pyrogen contaminations in pharmaceuticals.

Type of PCR

Digital PCR (dPCR)

Components

• NAT-MAT® Mix (lyophilized)
• Positive Control DNA (lyophilized)
• dPCR grade Water

Package Size

Cat. No. 58-0503: 96 reactions

Result Evaluation

Absolute Quantification

Required Lab Device and Consumables

• QIAcuity® digital PCR system
• QIAcuity® Nanoplate 8.5k 96-well
• QIAcuity® OneStep Advanced Probe Kit
• Nanoplate tray
• Nanoplate seal
• 1.5 ml reaction tubes
• Microcentrifuge for 1.5 ml PCR tubes
• Vortex mixer
• Pipettes with corresponding filter tips
• Pipetting robot (optional, e.g. QIAgility® with nanoplate adapter)

Shipment, Shelf Life, and Storage

Shipment at ambient temperature. The expiry date of the unopened package is marked on the package label. The kit components must be stored at +2 to +8°C until use. The rehydrated components must be stored at ≤ -18°C for a maximum of one month.

Do you prefer an all-in-one system, that provides all necessary components for performing MAT?
Have a look at NAT-MAT® All-in-One System that consists of NAT-MAT® Cells, NAT-MAT® Extraction and NAT-MAT® dPCR.

Minerva Biolabs의 모든 제품을 만나보세요!

Cell Culture Contamination Control

DNA / RNA Extraction

PCR Reagents & Enzymes

Pharma / ATMP

Decontamination Reagents for PCR Labs

Water Contamination Testing

Proficiency Testing Program 2024

Contract Manufacturing

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